Primer database

NES / nes / Nes: candidate comparison

Every NES / nes / Nes primer pair on file, overlaid on one shared exon axis, where each design actually binds, relative to the others.

⚠ 2pair(s) only mapped onto a different isoform than the one shown above, so they can't be plotted on this shared axis: PMID 36684426, PMID 39916688

Source	Organism	Amplicon	Tm	Specificity
PMID 38235293	Danio rerio	89 bp	63.9 °CF 63.6 / R 64.3	specific	View →
PMID 36684426	Homo sapiens	94 bp	64.1 °CF 64.1 / R 64.1	specific	View →
PMID 39916688	Mus musculus	79 bp	63.6 °CF 63.7 / R 63.6	specific	View →

Using these primers?

Analyse your qPCR run with LinRegPCR efficiency correction and a MIQE 2.0 report.