Paste the relative quantity and Cq of each point in your dilution series to get the standard curve slope, R², and amplification efficiency.
Quantity is relative concentration, higher means more concentrated (e.g. 1, 0.1, 0.01, 0.001 for a 10-fold serial dilution). At least 3 points. Values separated by commas or spaces, one pair per line.
Efficiency is derived from the slope of Cq plotted against log₁₀(relative quantity): E = 10^(−1/slope) − 1. A slope of −3.32 corresponds to 100% efficiency (an exact doubling each cycle); shallower slopes indicate lower efficiency.
This requires running a full dilution series, an extra plate and extra time. Ampliova's primary method skips that step: LinRegPCR (Untergasser et al. 2021) derives an equivalent per-reaction efficiency directly from the exponential phase of each amplification curve you already have, no separate standard curve needed. Read how it works
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