qPCR efficiency calculator
Got a dilution series? Enter the dilution and the Cq of each level, and you'll get the standard curve slope, R², and amplification efficiency.
Level 1 is the most concentrated: 1 for undiluted, or 1:5 if the series starts from a diluted cDNA.
Put the replicates of a level in the same cell, separated by spaces. Enter moves to the next level. You can paste a column straight from Excel: it fills the levels below.
Efficiency is derived from the slope of Cq plotted against log₁₀(relative quantity): E = 10^(−1/slope) − 1. A slope of −3.32 corresponds to 100% efficiency (an exact doubling each cycle); shallower slopes indicate lower efficiency.
The catch is that it takes a full dilution series: an extra plate, and extra time. Ampliova's main method skips it. LinRegPCR (Untergasser et al. 2021) reads an equivalent per-reaction efficiency straight off the exponential phase of the amplification curves you already have, so there's no separate standard curve to run. Read how it works
If you do run a dilution series, Ampliova fits it from the plate file too, gene by gene, with the 95% interval on the efficiency, and any batch can use that value instead of LinRegPCR. Standard curves in Ampliova
This page gives you the one number. Ampliova runs the whole efficiency-corrected analysis around it. Try it free