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qPCR Primer Database

Published qPCR primer pairs with the efficiency each source reported, exon-level mapping on Ensembl transcripts and an off-target specificity check.

1094

Primer pairs

874

Genes

15

Organisms

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22 primer pairs · filtered from 1094

Gene ↑OrganismAmpliconSourceMappingMeasurementsMean E
Casp1Mus musculus115 bpPublicationVerified✓ Specific——
Casp1Rattus norvegicus193 bpPublicationVerified✓ Specific——
CASP1Homo sapiens53 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus94 bpPublicationVerified✓ Specific——
Il1b⇄ compare (4)Rattus norvegicus110 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus89 bpPublicationVerified✓ Specific——
Il1b⇄ compare (4)Rattus norvegicus79 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus116 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus290 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus189 bpPublicationVerified✓ Specific——
Il1b⇄ compare (4)Rattus norvegicus85 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus104 bpPublicationVerified✓ Specific——
Il1b⇄ compare (7)Mus musculus220 bpPublicationVerified✓ Specific——
Il1b⇄ compare (4)Rattus norvegicus181 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens186 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens129 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens264 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens156 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens198 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens111 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens78 bpPublicationVerified✓ Specific——
IL1B⇄ compare (8)Homo sapiens132 bpPublicationVerified✓ Specific——

Using primers from this database? Analyse your qPCR run with LinRegPCR efficiency correction and a MIQE 2.0 report. Analyse your data →