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qPCR Primer Database

Published qPCR primer pairs with the efficiency each source reported, exon-level mapping on Ensembl transcripts and an off-target specificity check.

1094

Primer pairs

874

Genes

15

Organisms

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17 primer pairs · filtered from 1094

Gene ↑OrganismAmpliconSourceMappingMeasurementsMean E
Actb⇄ compare (8)Mus musculus171 bpPublicationVerified✓ Specific——
Actb⇄ compare (2)Rattus norvegicus93 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus145 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus287 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus104 bpPublicationVerified✓ Specific——
Actb⇄ compare (2)Rattus norvegicus181 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus83 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus147 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus198 bpPublicationVerified✓ Specific——
Actb⇄ compare (8)Mus musculus154 bpPublicationVerified✓ Specific——
ACTBGallus gallus151 bpPublicationVerified✓ Specific——
ACTB⇄ compare (4)Homo sapiens131 bpPublicationVerified✓ Specific——
ACTB⇄ compare (4)Homo sapiens121 bpPublicationVerified✓ Specific——
ACTBMus musculus101 bpPublicationVerified✓ Specific——
ACTB⇄ compare (4)Homo sapiens205 bpPublicationVerified✓ Specific——
ACTB⇄ compare (4)Homo sapiens118 bpPublicationVerified✓ Specific——
ACTG1Capra hircus157 bpPublicationVerified✓ Specific1?1.956

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