★ Founders price. 40% off the first two years of an annual plan started by June 30, 2027, then the standard price. See the plans →
← Primer database

Primer database

Myh7

myosin, heavy polypeptide 7, cardiac muscle, beta

Mus musculus

PublicationMarigny et al. 2026

Sequences

Forward (5′→3′)

AGGTGTGCTCTCCAGAATGG

Reverse (5′→3′)

CAGCGGCTTGATCTTGAAGT

Amplicon

139 bp

estimated from mapping

Primer Tm (°C)

ConditionsForwardReverseAverageΔ F/R
qPCR master mix63.362.062.61.3
Primer-BLAST default59.458.659.00.8

qPCR master mix: Owczarzy 2004/2008 · 250 nM primer · 50 mM Na⁺ · 3 mM Mg²⁺ · 0.8 mM dNTP

Primer-BLAST default: SantaLucia 1998 salt corr. · 50 nM primer · 50 mM Na⁺ · 1.5 mM Mg²⁺ · 0.6 mM dNTP

Bold, underlined base = 3′ end (polymerase extension point).

Primer QC

GC% · Forward

55.0%

GC% · Reverse

50.0%

Self-dimer ΔG · Fwd (any position)

-1.3 kcal/mol

Self-dimer ΔG · Fwd (3′ end)

-1.3 kcal/mol

Self-dimer ΔG · Rev (any position)

-1.6 kcal/mol

Self-dimer ΔG · Rev (3′ end)

No duplex found

Heterodimer ΔG (F×R, any position)

-1.5 kcal/mol

Heterodimer ΔG (F×R, 3′ end)

No duplex found

ΔG at 37 °C · SantaLucia 1998 NN parameters · < −6 kcal/mol = significant dimer risk · "3′ end" is restricted to duplexes anchored at a primer's own 3′ terminal base (polymerase-extendable); "any position" is the most stable duplex anywhere in the primer. Estimated screening value. For comprehensive dimer analysis, validate with Primer3.

Source

PMID

42063173(Europe PMC)

Citation

Marigny et al. 2026

Genomic mapping

Ensembl gene

ENSMUSG00000053093 ↗

NCBI Gene ID

140781

Assembly

GRCm39

Cross-check

ENSMUST00000228837
ENSMUST00000227518
ENSMUST00000153783
ENSMUST00000226424
ENSMUST00000576887
✓ENSMUST00000102803★
✓ENSMUST00000168485
✓ENSMUST00000412811
✓ENSMUST00000412813
✓ENSMUST00000412814
✓ENSMUST00000412815
✓ENSMUST00000412816
✓ENSMUST00000412818
✓ENSMUST00000412820
✓ENSMUST00000412825
✓ENSMUST00000412826
✓ENSMUST00000412828
✓ENSMUST00000412831
✓ENSMUST00000412832
✓ENSMUST00000412834
✓ENSMUST00000412835
✓ENSMUST00000412836
✓ENSMUST00000412837
✓ENSMUST00000412838
✓ENSMUST00000576886
~294bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.61bp~388bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.~418bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.~311bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.157bp28bp109bp93bp64bp99bp104bp139bp119bp150bp171bp310bp68bp88bp118bp124bp137bp~256bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.243bp~578bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.~407bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.~166bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.390bp127bp119bp197bp184bp166bp125bp~309bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.~204bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.126bp276bp96bp~135bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.127bp
exon presentprimer binds hereconfirmed amplicongap = exon skipped~bp = boundary varies
How do I read this diagram?

Each row is one transcript, aligned on a shared axis. A gap means that isoform skips this exonic region. Each row's box is drawn at that isoform's own real exon length, which can be narrower than the column (alternative splice site); hover a box for its exact size. A ~amber header bp means isoforms don't all agree on this region's boundary, while a plain header means they do. Navy blocks mark where the primers bind, and green (with a ✓ by the name) means this isoform has a confirmed working amplicon, not just exon overlap. A row with a “‹ exons” / “exons ›” hint instead of any box means that isoform's exons exist but sit outside the current scroll position (a truncated isoform or alternate transcription start site) — click the hint to jump to them. Click a row to load it below.

★ = Ensembl canonical transcript (reference model, not necessarily the most expressed isoform)

Showing: ENSMUST00000228837

104bp1386bp56bp2685bp209bp3578bp82bp4
Myh7 · ENSMUST00000228837No amplicon found on this isoform (intron_only / intron_only)

Off-target specificity

Specific

No paired forward+reverse off-target amplicon found against nt (whole genome/transcriptome). Screen requires >=2 total mismatches including >=2 within the last 5bp at the 3' end to clear a hit (NCBI Primer-BLAST-style stringency); hits with >=6 total mismatches are dropped as implausible.

Efficiency measurements

Ideal range:Dilution series 1.90–2.10·LinRegPCR 1.70–1.90·unknown method: not scored
No measurements yet for this primer pair.

Using these primers?

Analyse your qPCR run with LinRegPCR efficiency correction and a MIQE 2.0 report.

Analyse your data →