★ Founders price. 40% off the first two years of an annual plan started by June 30, 2027, then the standard price. See the plans →
← Primer database

Primer database

CFTR

CF transmembrane conductance regulator

Homo sapiens

PublicationTang et al. 2026

Sequences

Forward (5′→3′)

AAAAGGCCAGCGTTGTCTCC

Reverse (5′→3′)

AAACATCGCCGAAGGGCATTA

Amplicon

211 bp

as reported in the source

Primer Tm (°C)

ConditionsForwardReverseAverageΔ F/R
qPCR master mix64.864.064.40.9
Primer-BLAST default61.260.760.90.5

qPCR master mix: Owczarzy 2004/2008 · 250 nM primer · 50 mM Na⁺ · 3 mM Mg²⁺ · 0.8 mM dNTP

Primer-BLAST default: SantaLucia 1998 salt corr. · 50 nM primer · 50 mM Na⁺ · 1.5 mM Mg²⁺ · 0.6 mM dNTP

Bold, underlined base = 3′ end (polymerase extension point).

Primer QC

GC% · Forward

55.0%

GC% · Reverse

47.6%

Self-dimer ΔG · Fwd (any position)

-4.0 kcal/mol

Self-dimer ΔG · Fwd (3′ end)

0.1 kcal/mol

Self-dimer ΔG · Rev (any position)

-2.1 kcal/mol

Self-dimer ΔG · Rev (3′ end)

1.5 kcal/mol

Heterodimer ΔG (F×R, any position)

-2.5 kcal/mol

Heterodimer ΔG (F×R, 3′ end)

0.1 kcal/mol

ΔG at 37 °C · SantaLucia 1998 NN parameters · < −6 kcal/mol = significant dimer risk · "3′ end" is restricted to duplexes anchored at a primer's own 3′ terminal base (polymerase-extendable); "any position" is the most stable duplex anywhere in the primer. Estimated screening value. For comprehensive dimer analysis, validate with Primer3.

Source

PMID

42562793(Europe PMC)

Citation

Tang et al. 2026

Genomic mapping

Ensembl gene

ENSG00000001626 ↗

NCBI Gene ID

1080

Assembly

GRCh38

Cross-check

ENST00000673785
ENST00000446805
ENST00000699605
ENST00000468795
ENST00000689011
ENST00000600166
✓ENST00000699596
✓ENST00000699597
✓ENST00000699602
✓ENST00000648260
✓ENST00000649406
✓ENST00000649781
✓ENST00000003084★
✓ENST00000426809
✓ENST00000889206
✓ENST00000889207
✓ENST00000889208
✓ENST00000889209
✓ENST00000889210
✓ENST00000950799
✓ENST00001140285
46bp13.7kb169bp164.7kb85bp12.5kb55bp821bp102bp116bp233bp264bp~189bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.8.7kb109bp14.5kb215bp562bp111bp4.7kb109bp21.8kb~216bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.3.2kb90bp882bp164bp1.1kb126bp3.4kb247bp1.7kb93bp6.5kb~183bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.10.6kb~450bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.27.8kb95bp2.5kb87bp1.5kb~724bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.2.3kb129bp7.8kb38bp668bp251bp2.9kb80bp3.8kb151bp911bp~228bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.2.8kb~101bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.12.8kb~389bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.14.5kb156bp10.2kb~90bpThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.11.8kb173bp598bp106bp1.1kb~2.0kbThis is the outer span across every isoform's exon here. They don't all agree on the exact boundary (alternative splice site), so hover a row's own box below for its real length.47.1kb191bp
exon presentprimer binds hereconfirmed amplicongap = exon skipped~bp = boundary varies
How do I read this diagram?

Each row is one transcript, aligned on a shared axis. A gap means that isoform skips this exonic region. Each row's box is drawn at that isoform's own real exon length, which can be narrower than the column (alternative splice site); hover a box for its exact size. A ~amber header bp means isoforms don't all agree on this region's boundary, while a plain header means they do. Navy blocks mark where the primers bind, and green (with a ✓ by the name) means this isoform has a confirmed working amplicon, not just exon overlap. A row with a “‹ exons” / “exons ›” hint instead of any box means that isoform's exons exist but sit outside the current scroll position (a truncated isoform or alternate transcription start site) — click the hint to jump to them. Click a row to load it below.

★ = Ensembl canonical transcript (reference model, not necessarily the most expressed isoform)

Showing: ENST00000673785

46bp113.7kb169bp2164.7kb85bp337.6kb215bp4562bp111bp54.7kb109bp621.8kb216bp73.2kb90bp8882bp164bp91.1kb126bp103.4kb247bp111.7kb93bp126.5kb35bp13R
CFTR · ENST00000673785No amplicon found on this isoform (intron_only / exon_only)

Off-target specificity

Specific

No paired forward+reverse off-target amplicon found against nt (whole genome/transcriptome). Screen requires >=2 total mismatches including >=2 within the last 5bp at the 3' end to clear a hit (NCBI Primer-BLAST-style stringency); hits with >=6 total mismatches are dropped as implausible.

Efficiency measurements

Ideal range:Dilution series 1.90–2.10·LinRegPCR 1.70–1.90·unknown method: not scored
No measurements yet for this primer pair.

Using these primers?

Analyse your qPCR run with LinRegPCR efficiency correction and a MIQE 2.0 report.

Analyse your data →